Antioxidant defense and apoptotic effectors in ascorbic acid and β-glycerophosphate-induced osteoblastic differentiation
نویسندگان
چکیده
منابع مشابه
Human bone cell cultures in biocompatibility testing. Part II: effect of ascorbic acid, beta-glycerophosphate and dexamethasone on osteoblastic differentiation.
This work analyses the proliferation/differentiation behaviour of human bone marrow cells cultured in alpha-minimum essential medium supplemented with 10% foetal bovine serum (standard medium) and in the presence of ascorbic acid (AA, 50 microg ml(-1)), beta-glycerophosphate (betaGP, 10 mmol) and dexamethasone (Dex, 10 nmol) under selected experimental conditions. Cultures were compared concern...
متن کاملCharacterization of osteoblastic differentiation of stromal cell line ST2 that is induced by ascorbic acid.
The stromal cell line ST2, derived from mouse bone marrow, differentiated into osteoblast-like cells in response to ascorbic acid. Ascorbic acid induced alkaline phosphatase (ALPase) activity, the expression of mRNAs for proteins that are markers of osteoblastic differentiation, the deposition of calcium, and the formation of mineralized nodules by ST2 cells. We investigated the mechanism where...
متن کاملCombined effects of ascorbic acid and phosphate on rat VSMC osteoblastic differentiation.
BACKGROUND Ascorbic acid (AA) supplementation has been suggested to afford erythropoietin hyporesponsiveness and high levels of ferritin in haemodialysis (HD) patients. However, little is known about the possible side effects of this policy on vascular calcification (VC). VC, induced by a high-phosphate and uraemic milieu, is characterized by a passive deposition of calcium-phosphate (Ca-P) and...
متن کاملAscorbic acid monoglucoside as antioxidant and radioprotector.
Ascorbic acid monoglucoside (AsAG), a glucoside derivative of ascorbic acid, has been examined for its antioxidant and radioprotective abilities. AsAG neutralized 1, 1 diphenyl -2-picryl-hydrazyl (DPPH), a stable free radical in a concentration dependent manner thus indicating its antioxidant ability. AsAG protected mice liver tissues in vitro from peroxidative damage in lipids (measured as TBA...
متن کاملA novel bioactive osteogenesis scaffold delivers ascorbic acid, β-glycerophosphate, and dexamethasone in vivo to promote bone regeneration
Ascorbic acid, β-glycerophosphate, and dexamethasone have been used in osteogenesis differentiation medium for in vitro cell culture, nothing is known for delivering these three bioactive compounds in vivo. In this study, we synthesized a novel bioactive scaffold by combining these three compounds with a lysine diisocyanate-based polyurethane. These bioactive compounds were released from the sc...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Development, Growth & Differentiation
سال: 2011
ISSN: 0012-1592
DOI: 10.1111/j.1440-169x.2010.01232.x